Coding

Part:BBa_K1690003:Design

Designed by: Anna Sosnova, Pavel Zach   Group: iGEM15_Czech_Republic   (2015-09-17)


Mating pheromone (L. elongisporus)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The secretion tag was obtained by a genomic PCR from S. cerevisiae. The pheromone sequence was synthetized.


Source

This part is derived from BBa_K1690000. Using site-directed mutagenesis, we replaced the original pheromone sequence.

References